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Salazar-Mercado,Seir Antonio; Vega-Contreras,Nelson Alfonso. |
Abstract Cattleya trianae (Linden & Reichb.f., 1860), Colombian national flower, is in danger of extinction due to the destruction of its natural habitats and excessive collection for horticultural purposes. Therefore, in vitro culture is a tool for the conservation of threatened species. In this study we determined the most suitable culture medium for asimbytic seed germination and in vitro propagation of C. trianae. Initially, mature capsules were collected, the seeds were subsequently disinfected and seeded with the syringe method (Vendrame et al., 2007), to evaluate the effect of five media on the development of C. trianae after 20 weeks. The seedlings were transplanted and acclimated using different substrates. The best percentage (54.2%) of... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Organic additives; Coconut water; Pineapple juice; In vitro propagation. |
Ano: 2017 |
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122017000400544 |
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Costa,Sonia Helena Furtado; Santos,Regiane Rodrigues dos; Ferreira,Marcos Antônio Leal; Machado,Vanessa Porto; Rodrigues,Ana Paula Ribeiro; Ohashi,Otávio Mitio; Figueiredo,José Ricardo de. |
The present study investigated the efficiency of saline solution and coconut water solution in the preservation of goat preantral follicles enclosed in ovarian tissue, at different temperatures and for different incubation periods. At the slaughterhouse, the ovarian pair was divided into 19 fragments; one ovarian fragment was immediately fixed for histology (control-time zero). The other 18 ovarian fragments were preserved in both solutions at 4ºC, 20ºC or 39ºC for 4 h, 12 h or 24 h. The histological analysis showed that the storage of ovarian fragments in both solutions at 4ºC for up to 24 h kept the percentage of normal preantral follicles similar to the control values. In contrast, preservation at 20°C or 39ºC, in either solution, reduced significantly... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Preservation; Goat; Preantral follicles; Coconut water; Saline solution. |
Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962002000600009 |
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BISPO,VANDERSON S.; DANTAS,LUCAS S.; CHAVES FILHO,ADRIANO B.; PINTO,ISABELLA F.D.; SILVA,RAILMARA P. DA; OTSUKA,FELIPE A.M.; SANTOS,RODRIGO B.; SANTOS,ALINE C.; TRINDADE,DANIELLE J.; MATOS,HUMBERTO R.. |
ABSTRACT Hepatic disorders such as steatosis and alcoholic steatohepatitis are common diseases that affect thousands of people around the globe. This study aims to identify the main phenol compounds using a new HPLC-ESI+-MS/MS method, to evaluate some oxidative stress parameters and the hepatoprotective action of green dwarf coconut water, caffeic and ascorbic acids on the liver and serum of rats treated with ethanol. The results showed five polyphenols in the lyophilized coconut water spiked with standards: chlorogenic acid (0.18 µM), caffeic acid (1.1 µM), methyl caffeate (0.03 µM), quercetin (0.08 µM) and ferulic acid (0.02 µM) isomers. In the animals, the activity of the serum γ-glutamyltranspeptidase (γ-GT) was reduced to 1.8 I.U/L in the coconut... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coconut water; Ethanol; Oxidative stress; Phenolic compounds. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000301095 |
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Lima,G.L.; Santos,E.A.A.; Lima,L.F.; Luz,V.B.; Rodrigues,A.P.R.; Silva,A.R.. |
We compare protocols for the short-term preservation of collared peccarie's ovarian preantral follicles (PFs) by using phosphate buffered saline- (PBS) or powdered coconut water- (ACP(r)) based medium. For morphology analysis each pair of ovaries collected from six females was divided into nine fragments. One fragment was destined for morphology analysis (histology and transmission electron microscopy - TEM), constituting the control group and the other fragments were placed in tubes with PBS or ACP(r), packed in 5 L Styrofoam boxes, stored for 4h, 12h, 24h, and 36h, and then analyzed. For viability analysis a pair of ovaries from two additional females was divided into nine fragments; one fragment was immediately destined for viability analysis (Trypan... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Collared peccaries; Preantral follicles; Coconut water; Ovary; Oocytes. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000601623 |
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Zahara,Meutia; Datta,Avishek; Boonkorkaew,Patchareeya; Mishra,Abha. |
ABSTRACT The effects of various sucrose concentrations as carbon source and natural additives in different media on plantlet growth of Phalaenopsis hybrid 'Pink' were studied. Plantlets were cultured on two media (Murashige and Skoog [MS] and Vacin and Went [VW]) supplemented with 0, 10, 20, 30 and 40 g L-1 sucrose either with 0, 10 and 20% (v/v) coconut water (CW) or carrot juice (CJ) as natural additives. After four months of culture, the combination of sucrose and CW supplemented with both media affected plantlet growth where most of the plantlets showed slow growth and survival frequency (0-80%) with increasing concentrations of CW in all sucrose concentrations. However, plantlet growth on both media containing only 20 g L-1 sucrose without CW was... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Carrot juice; Coconut water; Natural additives; Phalaenopsis. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100501 |
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